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TS Metabolomics

COST ACTION Training School – Provisional Program:

Metabolite Profiling Approaches in Microorganisms and Higher Plants.

Venue: The school of Biological Sciences, Royal Holloway University of London, Egham Hill, Egham, Surrey, TW20 0RB. UK.

Course coordinator: Dr Paul Fraser

 

Training School Organizers:

Heribert WARZECHA and Paul FRASER (COST Action FA1006)

Gustavo M. DE BILLERBECK and Matthias WÜST (COST Action FA0907)

 

Date: 19th to 22rd March  2012.

 

Aims and objectives of the course: The course is directed towards postgraduate (Master and PhD) level as well as researchers not familiar with the field but where their research would benefit from the technology if the expertise was available.

The course will provide (i) an overview of the Metabolite profiling approaches used in the characterisation of microbial and higher plant studies in metabolic biology and (ii) first hand practical experience in the quenching of metabolism, extraction of metabolites, chromatographic separations and detection methods (e.g. hardware platforms) and data analysis.

 

Schedule

 

Monday 19th March:

09.00 to 9.15:  Welcome by Dr Fraser.

09.15 to 12.00 Introduction to metabolite profiling approaches (i) experimental design, (ii) tissue standardisation, (iii) quenching, (iv) extraction, (v) hardware platforms (including chromatography) and (vi) data analysis.

12.00 noon to 13.00: Lunch

13.00 to 15.00: Practical sessions

Group A: Microbial quenching and extraction

Group B: Homogenisation and extraction of metabolites from plant tissues.

15.00 to 15.30: Coffee break

15.30 to 17.30: Practical sessions

Group A: Homogenisation and extraction of metabolites from plant tissues

Group B: Microbial quenching and extraction.


Tuesday 20th March:

9.00 to 9.30: Intro/overview of the days practical.

9.30 to 12.30: Practical session

Group A: Derivatisation and GC-MS

Group B: LC-MS and LC-PDA.

12.30 noon to 13.30: Lunch

13.30 to 16.30: Practical session

Group A: LC-MS and LC-PDA

Group B: Derivatisation and GC-MS.

 

Wednesday 21st March:

9.00 to 12.30:

Expert talks showing the application of metabolite profiling

One talk by Bruker providing a hardware manufactures perspective

12.30 noon to 13.30: Lunch

14.00 to 17.00: Trip to Syngenta Jealott’s Hill site.

 

Thursday 22nd March

9.00 to 12.30: Data analysis

Basic statistical approaches

Multivariate PCA approaches

Display over bespoke pathways

Correlation analysis

13.00 to 14.00: Lunch

13.30 to 15.00: Question and answer session.

 

 

COST

EUCOST is supported by the EU RTD Framework Programme

EUESF provides the COST Office through a European Commission contract

EUThe Council of the European Union provides the COST Secretariat